Syllabus(Master’s Program)

Master’s Program

No. Title Instructor Credit Semester Notes
Reviews for Applied Life Sciences Professors 2 Apr-Sep Compulsory
Career Design in the Field of Applied Life Sciences Kohki AKIYAMA
(representative)
1 Year-round Compulsory
Scientific Presentation for Applied Life Sciences All faculty members 1 Oct-Mar Compulsory
Global Presentation for Applied Life Sciences Joseph Rodrigue 1 Oct-Mar Compulsory
Applied Bioscience Seminar A All faculty members 1 Apr-Sep Compulsory
Applied Bioscience Seminar B All faculty members 1 Oct-Mar Compulsory
Applied Bioscience Seminar C All faculty members 1 Apr-Sep Compulsory
Applied Bioscience Seminar D All faculty members 1 Oct-Mar Compulsory
Research Project in Applied Life Sciences A All faculty members 3 Apr-Sep Compulsory
Research Project in Applied Life Sciences B All faculty members 3 Oct-Mar Compulsory
Research Project in Applied Life Sciences C All faculty members 3 Apr-Sep Compulsory
Research Project in Applied Life Sciences D All faculty members 3 Oct-Mar Compulsory
Topics in Fermentation Chemistry Michihiko KATAOKA,
Masao KISHIDA
1 Oct-Mar
Topics in Bioresource Cycle Engineering Tatsuji SAKAMOTO,
Mitsuhiro UEDA
1 Apr-Sep
Topics in Bioactive Natural Products Kohki AKIYAMA,
Kenji KAI
1 Apr-Sep
Topics in Biological Macromolecules Takashi INUI,
Osamu ISHIBASHI
1 Oct-Mar
Topics in Nutrition Biochemistry and Functional Foods Ryoichi YAMAJI,
Naoki HARADA
1 Apr-Sep
Topics in Food Material Chemistry Naoya KASAI,
Mitsugu AKAGAWA
1 Oct-Mar
Topics in Applied Microbiology Takashi KAWAGUCHI,
Jun-ichi SUMITANI,
Shuji TANI
1 Apr-Sep
Topics in Bioorganic Chemistry Shinji TANIMORI,
Motohiro SONODA
1 Oct-Mar
Topics in Biophysical Chemistry Shinichi KITAMURA 1 Apr-Sep
Topics in Molecular and Cellular Biology Kenji SUGIMOTO 1 Oct-Mar
Topics in Functional Genomics Daisaku OHTA,
Atsushi OKAZAWA
1 Oct-Mar
Topics in Economic Botany Koh AOKI,
Masamichi OHE,
Yoshiyuki OGATA
1 Oct-Mar
Topics in Plant Breeding and Propagation Shuji YOKOI,
Takahiro TEZUKA,
Hajime FURUKAWA
1 Oct-Mar
Topics in Plant Production Sciences and Postharvest Physiology Shuji SHIOZAKI,
Yube YAMAGUCHI
1 Apr-Sep
Topics in Plant Production Practices Syuji SHIOZAKI,
Motoaki TOJO,
Hajime FURUKAWA,
Teruo WADA
1 Oct-Mar intensive course
Topics of Plant Biotechnology Koh AOKI,
Motoaki TOJO,
Yube YAMAGUCHI
1 Oct-Mar
Topics in Crop Ecophysiology Junpei TAKANO 1 Oct-Mar
Topics in Plant Developmental Biology and Physiology Yoshihiro IMAHORI 1 Oct-Mar
Topics in Plant Molecular Breeding Nozomu KOIZUMI,
Keiichiro MISHIBA
1 Apr-Sep
Topics in Plant Pathology Satoshi OHKI,
Motoaki TOJO,
Tomofumi MOCHIZUKI
1 Apr-Sep
Topics in Bioinformatics Koh AOKI,
Takashi INUI,
Daisaku OHTA,
Osamu ISHIBASHI,
Yoshiyuki OGATA
2 Apr-Sep
Selected Topics in Biological Sciences A TBD 1 Year-round intensive course
Selected Topics in Biological Sciences B TBD 1 Year-round intensive course
Selected Topics in Biological Sciences C TBD 1 Year-round intensive course
Selected Topics in Biological Sciences D TBD 1 Year-round intensive course

Notes

1. You must obtain at least a total of 30 credits, comprised of 22 credits for compulsory subjects, including ‘Research Integrity A’ of the Graduate School Common Courses, and 8 credits for elective subjects.

2. The credits for elective subjects include ‘Technology-based-Entrepreneurship Course’ of the Graduate School Common Courses.

3. If considered necessary by the academic advisor, you may have to take the department’s introductory courses. They will not be counted in the credits required for completion.
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Master’s Program

Title Reviews for Applied Life Sciences
Instructors Professors
Course Objectives This course focuses on advanced knowledge and topics in biological chemistry, bioscience and informatics, and plant bioscience to improve education and research activities.
Description and program Applied life science will be comprehensive subject clarifying various functions and life processes occurring in all forms of life (animals, plants, and microbes) on earth and contributing to the development of bioscience, biotechnology and applied technology. This subject is a basic compulsory subject. Professors in the division of Applied life sciences will present this course and aim to develop students who can understand the scientific role of this division and the social aspects. Lectures include the following topics: 1. Biological chemistry, 2. Plant biosciences.

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Title Career Design in the Field of Applied Life Sciences
Instructors Kohki AKIYAMA (representative)
Course Objectives Introduction to the cutting-edge and diverse fields of applied life sciences.
Description and program Some examples of prominent research projects are reported by outstanding researchers in companies and public research institutes. We aim to develop students who can learn problem-finding/solving skills in the field of applied life sciences systematically through discussion with researchers and engineers belonging to public research institutes or companies. Visits to institutes are also planned in order to experience firsthand current research and development activities.

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Title Scientific Presentation for Applied Life Sciences
Instructors All faculty members
Course Objectives Student will be able to apply knowledge of the research process to design, complete and present research projects.
Description and program The main focus of this course is to expose students, who have a background in applied life science, to the research process. Students will demonstrate their understanding of the research process by designing and completing an individual science research project.

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Title Global Presentation for Applied Life Sciences
Instructors Joseph Rodrigue
Course Objectives English listening comprehension, writing scientific abstracts and making presentations
Description and program The main focus of the course will be listening comprehension, but we will also discuss writing abstracts and creating slide presentations.

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Title Applied Bioscience Seminar A
Instructors All faculty members
Course Objectives The objective of course is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. To achieve this objective, student will work together in education and research related to the bioscience of animals, plants, and microorganisms in order to develop useful resources and to develop talented researchers who will make further contributions to the development of new biotechnologies.
Description and program Applied life sciences aims to research various functions and life processes occurring among animals, plants, and microorganisms by fully utilizing bioscience and biotechnology techniques. Our objective is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. In this course students are required to systematically develop project designs in applied life science, and to carry out the project. They are also required to acquire fundamental knowledge related to biological chemistry through bibliographic survey and discussion with other students and instructors.

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Title Applied Bioscience Seminar B
Instructors All faculty members
Course Objectives The objective of course is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. To achieve this objective, student will work together in education and research related to the bioscience of animals, plants, and microorganisms in order to develop useful resources and to develop talented researchers who will make further contributions to the development of new biotechnologies.
Description and program Applied life sciences aims to research various functions and life processes occurring among animals, plants, and microorganisms by fully utilizing bioscience and biotechnology techniques. Our objective is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. In this course students are required to systematically develop project designs in applied life science, and to carry out the project. They are also required to acquire fundamental knowledge related to biological chemistry through bibliographic survey and discussion with other students and instructors.

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Title Applied Bioscience Seminar C
Instructors All faculty members
Course Objectives The objective of course is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. To achieve this objective, student will work together in education and research related to the bioscience of animals, plants, and microorganisms in order to develop useful resources and to develop talented researchers who will make further contributions to the development of new biotechnologies.
Description and program Applied life sciences aims to research various functions and life processes occurring among animals, plants, and microorganisms by fully utilizing bioscience and biotechnology techniques. Our objective is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. In this course students are required to systematically develop project designs in applied life science, and to carry out the project. They are also required to acquire fundamental knowledge related to biological chemistry through bibliographic survey and discussion with other students and instructors.

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Title Applied Bioscience Seminar D
Instructors All faculty members
Course Objectives The objective of course is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. To achieve this objective, student will work together in education and research related to the bioscience of animals, plants, and microorganisms in order to develop useful resources and to develop talented researchers who will make further contributions to the development of new biotechnologies.
Description and program Applied life sciences aims to research various functions and life processes occurring among animals, plants, and microorganisms by fully utilizing bioscience and biotechnology techniques. Our objective is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. In this course students are required to systematically develop project designs in applied life science, and to carry out the project. They are also required to acquire fundamental knowledge related to biological chemistry through bibliographic survey and discussion with other students and instructors.

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Title Research Project in Applied Life Sciences A
Instructors All faculty members
Course Objectives Students are required to acquire basic, practical and applicable skills necessary in planning research related to the academic field of applied life science. In addition, they are required to conduct literature searches related to their individual research theme and to discuss the status, significance and development of their study with other students and the instructors.
Description and program Applied life sciences aims to research various functions and life processes occurring among animals, plants, and microorganisms by fully utilizing bioscience and biotechnology techniques. Our objective is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. In this course students are required to systematically develop project designs in applied life science, and to carry out the project. They are also required to acquire fundamental knowledge related to biological chemistry through bibliographic survey and discussion with other students and instructors. Each student designs a research plan, including the purpose and methods, evaluates the research progress overtime, and furthermore discusses the plan with the supervisors in order to make presentation and publication about the result of the research.

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Title Research Project in Applied Life Sciences B
Instructors All faculty members
Course Objectives Students are required to acquire basic, practical and applicable skills necessary in planning research related to the academic field of applied life science. In addition, they are required to conduct literature searches related to their individual research theme and to discuss the status, significance and development of their study with other students and the instructors.
Description and program Applied life sciences aims to research various functions and life processes occurring among animals, plants, and microorganisms by fully utilizing bioscience and biotechnology techniques. Our objective is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. In this course students are required to systematically develop project designs in applied life science, and to carry out the project. They are also required to acquire fundamental knowledge related to biological chemistry through bibliographic survey and discussion with other students and instructors. Each student designs a research plan, including the purpose and methods, evaluates the research progress overtime, and furthermore discusses the plan with the supervisors in order to make presentation and publication about the result of the research.

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Title Research Project in Applied Life Sciences C
Instructors All faculty members
Course Objectives Students are required to acquire basic, practical and applicable skills necessary in planning research related to the academic field of applied life science. In addition, they are required to conduct literature searches related to their individual research theme and to discuss the status, significance and development of their study with other students and the instructors.
Description and program Applied life sciences aims to research various functions and life processes occurring among animals, plants, and microorganisms by fully utilizing bioscience and biotechnology techniques. Our objective is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. In this course students are required to systematically develop project designs in applied life science, and to carry out the project. They are also required to acquire fundamental knowledge related to biological chemistry through bibliographic survey and discussion with other students and instructors. Each student designs a research plan, including the purpose and methods, evaluates the research progress overtime, and furthermore discusses the plan with the supervisors in order to make presentation and publication about the result of the research.

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Title Research Project in Applied Life Sciences D
Instructors All faculty members
Course Objectives Students are required to acquire basic, practical and applicable skills necessary in planning research related to the academic field of applied life science. In addition, they are required to conduct literature searches related to their individual research theme and to discuss the status, significance and development of their study with other students and the instructors.
Description and program Applied life sciences aims to research various functions and life processes occurring among animals, plants, and microorganisms by fully utilizing bioscience and biotechnology techniques. Our objective is to develop applied technologies through collaboration and competition in various domains of advanced life sciences. In this course students are required to systematically develop project designs in applied life science, and to carry out the project. They are also required to acquire fundamental knowledge related to biological chemistry through bibliographic survey and discussion with other students and instructors. Each student designs a research plan, including the purpose and methods, evaluates the research progress overtime, and furthermore discusses the plan with the supervisors in order to make presentation and publication about the result of the research.

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Title Topics in Fermentation Chemistry
Instructors Michihiko KATAOKA, Masao KISHIDA
Course Objectives Recent topics in fermentation chemistry and technology are introduced.
Description and program Fermentation (microbial production of useful compounds) is an important technology for humans. Microbial metabolism in fermentation consists of a large number of chemical reactions catalyzed by enzymes. Ongoing topics include the fundamental concepts of microbiology from the viewpoint of applied microbiology (introductory); the intermediary metabolism of carbohydrates, nitrogen compounds, lipids and hydrocarbons in the interrelationship between microbial physiology and biochemical processes; fermentative foods and the fermentation industry; the characteristics of microorganisms and the investigative methods pertaining to them (including microorganisms in the fermentation industry); utilization of microbial enzymes and genes; and developments in the production of useful compounds by microbial processes.

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Title Topics in Bioresource Cycle Engineering
Instructors Tatsuji SAKAMOTO, Mitsuhiro UEDA
Course Objectives The aim of this subject is to study new engineering and technology to reduce the negative environmental impact of modern society.
Description and program The aim of this subject is to understand the viewpoints of macro- and micro-chemistry for useful bio-resources and the basic concepts for recycling use and technologies. Topics include the balance of energy production and environmental damage, fusion technologies for useful and efficient biomass-energy production, political and social problems among private companies, multiuse technology development for waste to achieve zero emission, isolation of useful materials from bio-resources, basic research into enzymes and biological activity for the production of useful products from bio-resources, and environmental cleaning technologies.

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Title Topics in Bioactive Natural Products
Instructors Kohki AKIYAMA, Kenji KAI
Course Objectives In this course we aim to better understand the role of small molecules in ecological phenomena, and to acquire knowledge about the methods of isolation and structure determination of bioactive natural products.
Description and program An array of structurally diverse small molecules plays an important role in plant-microbe and microbe-microbe interactions. This class will describe the discovery, isolation and structure elucidation of natural products that act as signaling molecules in symbiotic and pathogenic interactions of plants and microbes. In addition, biosynthetic pathway and regulation of their production as well as molecular, physiological and morphological responses to the small molecules are also discussed.

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Title Topics in Biological Macromolecules
Instructors Takashi INUI, Osamu ISHIBASHI
Course Objectives The objectives of this course are to understand the molecular structures and functions of biological macromolecules such as proteins and nucleic acids. In addition, we will study biomedical application of the biological macromolecules, such as drug delivery systems (DDS) and molecular diagnostics.
Description and program This course consists of the following topics:
1. Drug delivery systems using intravital transporter proteins
2. Drug discovery research including genomic drug discovery
3. Computer-aided drug design
4. Neurodegenerative disorders induced by structurally abnormal proteins
5. Molecular mechanism of Huntington’s disease (polyglutamine disease)
6. Mechanisms of enzyme reaction based on structural information
7. Biological application of a large synchrotron radiation facility (SPring-8)
8. Non-coding RNAs (e.g., microRNAs) as therapeutic and diagnostic targets.

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Title Topics in Nutrition Biochemistry and Functional Foods
Instructors Ryoichi YAMAJI, Naoki HARADA
Course Objectives The aim of this course is to understand the functional roles of food ingredients, including nutrients (vitamins etc.) and non-nutrients (polyphenols etc.) to prevent and improve locomotive syndrome and metabolic syndrome called lifestyle-related diseases for the promotion of human health.
Description and program The molecular mechanisms by which osteoporosis, sarcopenia, obesity, and diabetes develop and the relationship between their diseases and lifestyle are studied. Ongoing topics include functional roles of food ingredients, such as polyphenols, which suppress the development and progression of osteoporosis, disuse atrophy, obesity, diabetes mellitus, breast cancer, prostate cancer, and so on, in addition to the molecular nutrition of micronutrients, including retinol (vitamin A), carotenoids, and vitamin D, and so on.

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Title Topics in Food Material Chemistry
Instructors Naoya KASAI, Mitsugu AKAGAWA
Course Objectives The investigation, application, and production of components of food materials or agricultural products. The food chemical, biochemical, and bio-industrial fields will be studied. Learning and understanding methods of investigation, planning and obtaining related knowledge are further objectives.
Description and program Chemical, biochemical and bio-molecular implications and applications in the fields of food material chemistry are studied. Recent topics in the fields are studied from the view of food chemical, biochemical, and applied enzymatic chemical fields. Ongoing topics include methods for the study of food chemicals and chemistry, and exploring food functions and the potential of food materials. Applications and developments in agricultural and biological industries or bio-resources are also included.

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Title Topics in Applied Microbiology
Instructors Takashi KAWAGUCHI, Jun-ichi SUMITANI, Shuji TANI
Course Objectives To understand the diversity and potential of microbial functions, and discuss the future prospects of their application.
Description and program It is no exaggeration to say that the diversity of microorganisms and their ability have unlimited potential. We take advantage of their abilities intentionally or unintentionally for a life of affluence. This class will describe the methodology of searching for and improving microorganisms’ abilities using recent examples, and will also discuss new techniques in applied microbiology and future development of filamentous fungi.
History and present situation of R & D for microbial utilization Specificity of filamentous fungi: Genetic biochemistry and research methodology Availability of filamentous fungi: Filamentous fungi as a factory of protein production
Topics in microbial utilization (1): Microbial cellulases and amylases
Topics in microbial utilization (2): Ethanol production from biomass
Topics in microbial utilization (3): Cellulase and amylase inhibitors
Topics in microbial utilization (4): Other useful enzymes and metabolites

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Title Topics in Bioorganic Chemistry
Instructors Shinji TANIMORI, Motohiro SONODA
Course Objectives Topics in Bioorganic Chemistry deals with recent advances in the fields of organic chemistry, bioorganic chemistry, chemical biology, medicinal chemistry, and pesticide chemistry. For instance, the design of drugs and pesticides, lead generation based on natural products and a chemical ecology approach, efficient and environmentally benign synthetic methodology directed toward green chemistry, combinatorial chemistry and high-throughput screening for efficient lead discovery, and discovery of drug candidates based on new nano-technology will be discussed.
Description and program 1. Introduction
2. Molecular design of small molecular bioactive compounds
3. Natural products and chemical ecology approach for lead generation
4. Cost-effective synthetic methods for green chemistry
5. Combinatorial chemistry and high-throughput screening
6. New nano-technology approach for the discovery of drug candidates
7. Chemical biology, chemical genetics, and chemical genomicsText: Not specified
Text book: Timely and appropriate information is provided
Achievement evaluation: Reports for announced assignments are evaluated for grade points

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Title Topics in Biophysical Chemistry
Instructors Shinichi KITAMURA
Course Objectives The objective of this course is to understand how biopolymers such as nucleotides, proteins, and polysaccharides function properly based on higher-order structures. In addition, we will study the application of these biopolymers in bionanotechnology.
Description and program This course consists of the following topics:
1. Structure and analysis of biopolymers(2)
2. Biochemical thermodynamics of biopolymers(1)
3. Function and higher-order structure of biopolymers(1)
4. Mechanism of functional properties of biopolymers(1)
5. Bionanotechnology and biopolymers(2)Text: Not specified
Text book: Timely and appropriate information is provided
Achievement evaluation: Reports for announced assignments are evaluated for grade points

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Title Topics in Molecular and Cellular Biology
Instructors Kenji SUGIMOTO
Course Objectives The goal of this course is to understand cellular functions, especially mitotic cell division processes, based on live cell imaging techniques using fluorescent proteins.
Description and program This course consists of the following topics:
1. Introduction
2. Chromatin and chromosome structures
3. Histone modification and epigenetics
4. Mitotic apparatus and chromosome segregation
5. Fluorescent proteins and chromophores
6. Visualization of protein-protein interactions using BiFC
7. Live cell imaging techniques and their applicationText: Not specified
Text book: Timely and appropriate information is provided
Achievement evaluation: Reports for announced assignments are evaluated for grade points

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Title Topics in Functional Genomics
Instructors Daisaku OHTA, Atsushi OKAZAWA
Course Objectives Students are expected to acquire basic academic skills to conduct life science research using genomics information.
Description and program Study basic strategies for holistic analyses including transcriptomics and metabolomics approaches.
Several important examples are selected from recent publications in functional genomics studies.
1. Introduction
2. Transcriptomics
3. Metabolomics (metabolic profiling and metabolic phenotypes)
4. Metabolomics (metabolite identification)
5. Metabolomics (gene function and pathway simulation)
6. Integrated analyses of transcriptomics and metabolomics
7. Industrial applicationsText: Not specified
Text book: Timely and appropriate information is provided
Achievement evaluation: Reports for announced assignments are evaluated for grade points

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Title Topics in Genomics of Plant Resources
Instructors Koh AOKI, Yoshiyuki OGATA
Course Objectives Students will acquire the latest knowledge of 1) genetic diversity, 2) artificially induced genetic diversity, 3) transcriptional variation, 4) gene transfer, 5) genomic data resources, 6) species-specific genes. Lectures will address the theories that are necessary for the inference of plant genome functions, and for the development of genomic resources.
Description and program Students are expected to learn detection methods and molecular mechanisms underlying transcriptional variation and gene transfer. They are expected to learn basics of network biology in order to infer the specific functions of plant genes.
Topics are as follows;
1. Genetic diversity of crops
2. Transcriptional variations.
3. Detection of gene transfer phenomena.
4. Mutagenized population
5. Public databases compiling genomics information
6. Identification of species-specific genes
7. Functions of species-specific genes
Text: Not specified
Reference Introduction to genomics (Arthur Lesk), Bioinformatics (David Mount)
Achievement evaluation: Reports for announced assignments are evaluated for grade points

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Title Topics in Plant Breeding and Propagation
Instructors Shuji YOKOI, Takahiro TEZUKA, Hajime FURUKAWA
Course Objectives Recent studies on plant breeding and propagation, and trends in the industry are introduced. In the field of plant breeding, the method for the application of progressive genomics to practical breeding is explained. In the field of plant propagation, the change of seed propagation to vegetative propagation in the practical production of seedlings and the importance of both propagation methods are explained. Further, new propagation methods and the conditions for their introduction into practice are addressed. In this course, specialists in plant breeding and propagation, with knowledge and techniques for practice, are developed.
Description and program Course outline, Selection by molecular marker, Application of molecular biology to breeding, Evolution of crops by genomic change, Characteristics of seed and vegetative propagation, New methods for plant propagation, Global trends in seedling production industry

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Title Topics in Plant Secondary Metabolites
Instructors Shuji SHIOZAKI, Yube YAMAGUCHI
Course Objectives Learning goal of this course is to acquire the knowledge of the function of secondary metabolism in plant and the role in human health. At the completion of this course, students will be able to 1) classify the plant secondary metabolites, 2) describe the biosynthesis of plant secondary metabolites, 3) describe the key role of plant secondary metabolites in plant, 4) describe how dietary plant secondary metabolites are absorbed, metabolized and disported in human body.
Description and program This course consists of the following topics:
1. Classification of phenolic compounds
2. Biosynthesis of flavonoids, stilbenes, hydroxycinnamates and phenolic acids.
3. Absorption and metabolism of dietary plant secondary metabolites
4. Useful terpenes and terpenoids: biosynthesis, physiological role and application
5. Useful alkaloids: biosynthesis, physiological role and application
6. Biotechnology for the production of plant secondary metabolites

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Title Topics in Plant Production Practices
Instructors Shuji SHIOZAKI, Motoaki TOJO, Hajime FURUKAWA, Teruo WADA
Course Objectives An understanding of effective plant management for higher yields of several crops, such as field and horticultural crops; medium preparation, fertilization, propagation, application of plant growth regulators, herbicides, insecticides and pesticides.
Description and program Effective crop management is based on good understanding of the physiological and morphological events of the plants. This course focuses on the following areas:
(1) The dependence of the growth and development of crops on N, P, and K fertilizer, including understanding of rooting systems and the chemical and biological properties and functionality of the rhizosphere and endophytes of crops
(2) CO2 assimilation of crops and translocation of the assimilates to each organ, such as leaf, stem, root and fruit regulation by plant nutrients and hormones
(3) Interaction between crops and microorganisms, especially focusing on various plant pathogens, such as viruses and fungi, including the possibility of manipulating plant disease resistance. Practical management technologies for several plant species, including field crops, horticultural crops, and model plants, are also introduced.

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Title Topics of Plant Biotechnology
Instructors Koh AOKI, Motoaki TOJO, Yube YAMAGUCHI
Course Objectives It is intended as an introduction to plant biotechnology for postgraduates in the field of breeding science, plant pathology and agricultural biology. Examples of molecular approaches to crop improvement and nutrition are discussed in many chapters. To understand both crop improvement and nutrition one must understand how genetic information is transmitted from one generation to the next, and how crop plants interact directly with numerous other organisms (pest, pathogens and symbiotic microbes). Intended Learning Outcomes: 1. To understand the potential applications of new technologies in the exploitation of plants. 2. To understand how knowledge of fundamental plant processes can be applied to drive biotechnology developments.
Description and program 1. The molecular basis of genetic modification and improvement of crops.
2. From classical plant breeding to modern crop improvement.
3. Plant-pathogenic bacteria and fungi cause many economic important diseases.
4. Classical strategies of crop protection against pathogen.
5. Development, productivity and sustainability of crop production.
6. Biotechnology will contribute to the rise of crop yields.
7. Genomics will dramatically transform crop improvement.

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Title Topics in Crop Ecophysiology
Instructors Junpei TAKANO
Course Objectives Students will acquire the latest knowledge of plant nutrition and physiology at molecular levels. The knowledge is a basement to improve crop production with limited mineral resources in various environments. Students will be required to read relevant chapters in a textbook “Biochemistry & Molecular Biology of Plants, Second Edition, Wiley Blackwell” and to introduce figures in the class.
Description and program 1, Guidance, Plant Nutrition
2, Nitrogen fixation 1
3. Nitrogen fixation 2
4. Uptake and metabolism of mineral nitrogen
5. Acquisition of phosphorus and potassium
6. Iron acquisition and aluminum tolerance
7, Summary

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Title Topics in Plant Developmental Biology and Physiology
Instructors Yoshihiro IMAHORI, Masamichi OHE
Course Objectives An understanding of basic plant physiology and the relationship to plant growth and development; role of soil microorganisms in crop yield and nutrient recycling, nutrient dynamics in several cropping systems and physiological/molecular mechanisms of plant adaptation to various environmental stresses.
Description and program This course focuses on the following areas:
(1) Legume-rhizobial associations with effective solar-driven N2-fixing systems in which atmospheric N2 is transformed into ammonia, without net CO2 emissions
(2) The contribution of rhizodeposition, roots remaining in the soil after crop harvest, on N and P dynamics in crop rotation systems, including understanding of root profiles and rhizosphere microorganisms focusing on nutrient recycling
(3) The regulation of lactic acid and ethanol fermentation, which regenerates NAD+ for the continuation of glycolysis in fruits and vegetables in response to low oxygen
(4) The role of fermentative metabolites on the physiology and quality of fruits and vegetables, in order to determine their tolerance to low oxygen and the potential of CA treatment.
Throughout this course, it is expected that practical technologies of crop production and post-harvest management will be understood well.

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Title Topics in Plant Molecular Breeding
Instructors Nozomu KOIZUMI, Keiichiro MISHIBA
Course Objectives Genetic information and DNA sequences have been rapidly increasing since the end of the last century. Genetic engineering technology has also improved. These have been applied for plant breeding and some are already commercialized. This subject will introduce modern breeding techniques using genetic information and engineering. Basic science and technology underlying the research and development of modern crops will also be addressed. Students will be required to read research articles and to present a summary of those papers after discussion in small groups.
Description and program The specific topics of this course are as follows:
1. What is molecular breeding?
2. Plant genome and post-genome research
3. Technology for introducing DNA in plant cells
4. Genetic transformation of various crops
5. Commercialization of genetic engineered plants
6. Marker- associated selection for plant breeding
7. Field of plant molecular breeding

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Title Topics in Plant Pathology
Instructors Satoshi OHKI, Motoaki TOJO, Tomofumi MOCHIZUKI
Course Objectives Students are expected to acquire advanced knowledge in plant pathology and related fields: molecular plant pathology for disease-resistant plants, pathogenicity, resistance and molecular plant breeding. Basic knowledge of plant pathology and microbiology is a prerequisite for this class.
Description and program This class deals with the pathogenicity of disease agents and resistance by plant hosts at the molecular level; gene expression in plant defense and stress resistance, signal transduction and pathogenicity-related chemicals, and genetics in pathogenicity. Topics include pathogenicity to plants by pathogens, such as viruses and soil-bone fungi, virulence of the pathogens to plant hosts and defense mechanisms by plant hosts to the pathogens, and viral infection and post-transcriptional gene silencing in plants against viral infection.

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Title Topics in Bioinformatics
Instructors Koh AOKI, Takashi INUI, Daisaku OHTA, Osamu ISHIBASHI, Yoshiyuki OGATA
Course Objectives Bioinformatics is computational analysis of the data generated by genome sequencing, array-based technologies, next-generation sequencing, proteomics, molecular-to-molecular interaction, polymorphism, and biomolecular networks. This lecture aims to understand and acquire the skills of bioinformatics for retrieving and applying useful information from biological and biochemical big-data.
Description and program Although the clearest method to determine the structure and the function of biomolecules is to carry out the direct experiments, it is far easier to estimate such structure and function by using DNA sequence of the molecules than to determine a function and structure experimentally. By designing bioinformatics approaches for such estimation, knowledge of the structure and the function is obtained, and furthermore, the integration of their knowledge leads to novel biological and biochemical insights. In this lecture, students acquire skills of advanced bioinformatics and practice its computational approaches. This lecture is given not only for students in a field of bioscience, but also for students in other fields.